High diversity of responses among Medicago truncatula lines to Phoma medicaginis infection.

Nourhene Maiza; Cheima Jabri; Nawres Zaidi; Khawla Rafik; Bilel Khiari; Naceur Djébali; Maria Teresa Sánchez-Ballesta; Mounawer Badri

doi:10.56027/JOASD.spiss082021

Authors

Nourhene Maiza
Cheima Jabri
Nawres Zaidi
Khawla Rafik
Bilel Khiari
Naceur Djébali
Maria Teresa Sánchez-Ballesta
Mounawer Badri

DOI:

https://doi.org/10.56027/JOASD.spiss082021

Keywords:

M. truncatula, lines, Phoma medicaginis, growth and pathogenic parameters

Abstract

Medicago truncatula is an omni-Mediterranean species grown as an annual forage legume. In addition to its small genome size and simple genetics, M. truncatula harbors several attributes, which make it an attractive model forage plant. In this study, we investigated the variation of responses in ten parental lines of M. truncatula to Phoma medicaginis infection. Plants were cultivated in the growth chamber under controlled conditions and were inoculated after two months with P. medicaginis. At harvest, 13 quantitative traits of growth and pathogenicity were measured. Results from ANOVA showed that the variation of analyzed parameters was explained by the effect of line. All measured parameters, except the root fresh weight, showed significant difference among the 10 studied lines. Most tolerant lines are those with the lowest ratios of the number of infected and dead leaves. Studied lines were clustered into three groups according to their responses to P. medicaginis infection. The most resistant TN6.18 line and most sensitive F83005.5 to P. medicaginis are useful for the exploration of physiological mechanisms and genetic determinants for M. truncatula tolerance to this constraint.

References

Angevain, M., Bernard, R., Bresson, F. (1983). Méthodes d’infection pour la sélection de la luzerne contre Phoma medicaginis. Malbr. &Roum. Agronomie 3, 911‒916.

Badri, M., Bouhaouel, I., Arraouadi, S., Taamalli, W., Huguet, T., Aouani, M.E. (2016). Variation in tolerance to drought among Tunisian populations of Medicago truncatula. Plant Genetic Resources 14(1), 41.

Barbetti, M.J. (1995). Resistance in annual Medicago species to Phoma medicaginis and Leptosphaerulina trifolii under field conditions. Australian Journal of Experimental Agriculture 35, 209‒214.

Djébali N. (2013). Aggressiveness and host range of Phoma medicaginis isolated from Medicago species growing in Tunisia. Phytopathologia Mediterranea 52, 3‒15.

Djébali, N., Mhadhbi, H., Jacquet, C., Huguet, T., Aouani, M.E. (2007). Involvement of hydrogen peroxide, peroxidase and superoxide dismutase in response of Medicago truncatula lines differing in susceptibility to Phoma medicaginis infection. Journal of Phytopathology 155, 633‒640.

Edmunds, L.K., Hanson, E.W. (1960). Host range, pathogenicity, and taxonomy of Ascochyta imperfecta. Phytopathology 50, 105‒108.

Ellwood, S.R., Kamphuis, L.G., Olivier, R.P. (2006). Identification of sources of resistance to Phoma medicaginis isolates in Medicago truncatula SARDI core collection accessions, and multigene differentiation of isolates. Phytopathology 96, 1330‒1336.

Gray, F.A., Fernandez, J.A., Horton, J.I. (1990). Variation among isolates of Phoma medicaginis var. medicaginis in spore production in vitro and symptom expression on excised leaves of alfalfa. Plant Disease 74, 668‒670.

Tivoli, B., Baranger, A., Sivasithamparam, K., Barbetti, M.J. (2006). Annual Medicago: from a model crop challenged by a spectrum of necrotrophic pathogens to a model plant to explore the nature of disease resistance. Annals of Botany 98, 1117‒1128.

Tlahig, S., Yahia, H., Loumerem, M. (2017). Agro-morphological homogeneity of Lucerne (Medicago sativa L. subsp. sativa) Half-sib progenies bred for outside oases conditions of Southern Tunisia. Journal of New Sciences 37, 2031–41.

Vadez, V., Rodier, F., Payre, H., Drevon, J.J. (1996). Nodule permeability and nitrogenase-linked respiration in bean genotypes varying in the tolerance to P deficiency. Plant Physiology and Biochemistry 35, 671‒678

Young, N.D., Udvardi, M. (2009). Translating Medicago truncatula genomics to crop legumes. Current Opinion in Plant Biology 12, 193–201.