Variation of Medicago sativa varieties tolerance to Phoma medicaginis infection.: M. sativa

Nawres Zaidi; Cheima Jabri; Nourhene Maiza; Khawla Rafik; Bilel Khiari; Naceur Djébali; Ndiko Ludidi; Mounawer Badri

doi:10.56027/JOASD.spiss092021

Authors

Nawres Zaidi
Cheima Jabri
Nourhene Maiza
Khawla Rafik
Bilel Khiari
Naceur Djébali
Ndiko Ludidi
Mounawer Badri

DOI:

https://doi.org/10.56027/JOASD.spiss092021

Keywords:

M. sativa, variety, Phoma medicaginis, quantitative parameters

Abstract

Due to its very important agronomic value and nutritional quality, Medicago sativa L. is considered as the queen of fodder and the first cultivated forage crop in the world. In field conditions, M. sativa is exposed to several biotic and/or abiotic constraints that affect its quality. In this regard, research is still underway to improve M. sativa resistance to many biotic stresses and, in this context, we analyzed the responses of a core collection of 10 varieties of M. sativa to Phoma medicaginis infection. Results from ANOVA showed that most growth parameters exhibited significant differences between the studied varieties. Nevertheless, only the number of healthy leaves among infection parameters varied significantly between the varieties. The local variety Gabès2355 exhibited the highest biomass. Positive correlations were found between the measured parameters. PCA based on the traits showing significant differences among the studied lines showed that the Gabès variety formed a separate group. Cluster analysis revealed that the studied varieties are classified into three major groups. The first group is formed by Gabès2353, the second group is composed of the Californian and El Hamma varieties, and the third group is constituted of the seven remaining varieties. Gabès2355 was the most tolerant to the Pm8 strain of P. medicaginis while Magna601 variety was the most susceptible. These two varieties will be useful to analyze the physiological and genetic determinants for M. sativa tolerance to P. medicaginis infection.

References

Badri, M., Bouhaouel, I., Arraouadi, S., Taamalli, W., Huguet, T., Aouani, M.E. (2016a). Variation in tolerance to drought among Tunisian populations of Medicago truncatula. Plant Genetic Resources 14(1), 41.

Badri, M., Toumi, G., Mahfoudh, S., Hessini, K., Abdelguerfi‐Laouar, M., Abdelguerfi, A., Aouani, M.E., Abdelly, C., Djébali, N. (2016b). Diversity of response to drought in a collection of lines of Medicago truncatula, M. ciliaris, and M. polymorpha. Crop Science 56(6), 3125–3132.

Benabderrahim, M.A., Haddad, M., Ferchichi, A. (2009). Diversity of lucerne (Medicago sativa L.) populations in South Tunisia. Pakistan Journal of Botany 41(6), 2851–61.

Chen, T., Yang, Q., Gruber, M., Kang, J., Sun, Y., Ding, W., Zhang, T., Zhang, X. (2012). Expression of an alfalfa (Medicago sativa L.) ethylene response factor gene MsERF8 in tobacco plants enhances resistance to salinity. Molecular Biology Reports 39(5), 6067–6075.

Djébali, N. (2013). Aggressiveness and host range of Phoma Medicaginis isolated from Medicago species growing in Tunisia. Phytopathologia Mediterranea 52(1), 3−15

Djébali, N., Aribi, S., Taamalli, W., Arraouadi, S., Aouani, M.E., Badri, M. (2013). Natural variation of Medicago truncatula resistance to Aphanomyces euteiches. European Journal of Plant Pathology 135(4), 831-843.

Samac, D.A., Willert, A.M., McBride, M.J., Kinkel, L.L. (2003). Effects of antibiotic-producing streptomyces on nodulation and leaf spot in alfalfa. Journal of Applied Soil Ecology 22(1), 55–66.

Tlahig, S., Yahia, H., Loumerem, M. (2017). Agro-morphological homogeneity evaluation of lucerne (Medicago.0 sativa L. subsp. sativa) half-sib progenies bred for outside oases conditions of southern Tunisia. Journal of New Sciences 37(3), 2031−2041.