Improving germination and growth performances of millet grown under saline conditions through seed priming

Hasna Ellouzi; Souhir Amraoui; Mohsen Hanana

doi:10.56027/JOASD.302023

Authors

Hasna Ellouzi
Souhir Amraoui
Mohsen Hanana CBBC

DOI:

https://doi.org/10.56027/JOASD.302023

Keywords:

Germination, Growth, Hormo-priming, Osmo-priming, Salt stress, Priming

Abstract

In the present study, the effect of seed priming by PEG (20%) and GA₃ (50 ppm) was investigated on germination performance, early seedling growth and water status of Pennisetum glaucum seedlings exposed to salt stress (100 mM NaCl). Our results showed that both types of seed treatments (PEG and GA₃) improved salt tolerance of millet, relatively to unprimed state. Although, some differences associated with agent nature were recorded. At germination stage and under salt stress, a significant increase in germination percentage (100% at the 6^th day of sowing) was recorded in seeds primed with GA₃. Additionally, they showed the highest Germination Rate Index (7.5) compared to non-primed seeds subjected to saline conditions. After germination has occured in the salt-stressed medium, seedlings from GA₃primed seeds exhibited the best ability to grow under saline conditions, as indicated by the highest root and coleoptile lengths. Furthermore, seed pre-treatment positively impacted biomass production, with seeds primed with GA3 showing the highest increase (193%) in biomass under saline conditions compared to non-primed seeds.In terms of water status, non-primed seedlings experienced a 50% reduction in water content under saline conditions. Seedlings from primed seeds, particularly those treated with GA₃, exhibited a significant increase in water content, with a remarkable 330% improvement compared to non-primed seedlings subjected to salinity stress.These findings demonstrate the effectiveness of seed pre-treatment, especially with GA₃, in enhancing the germination, growth, and water status of Pennisetum glaucum under saline conditions, offering potential strategies for improving crop resilience in saline environments.

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