Volatile compounds and bioactivities properties of Opuntia microdasys (Lehm.) and Opuntia macrorhiza (Engelm.) seeds

Hassiba Chahdoura; Asma El Ayeb; Mejdi Snoussi; Guido Flamini; Manel Ben M'hadheb; safia El-BOK; Lotfi Achour

doi:10.56027/JOASD.072024

Authors

Hassiba Chahdoura Unité de Recherche “Génomique, Biotechnologie et Stratégies Antivirales”, Institut Supérieur de Biotechnologie, Université de Monastir, BP74, Avenue Tahar Hadded, 5000 Monastir, Tunisia. https://orcid.org/0000-0002-5781-6946
Asma El Ayeb Laboratoire de Recherche “Bioressourses, Biologie Intégrative & Valorisation”, Institut Supérieur de Biotechnologie de Monastir, Tunisia.
Mejdi Snoussi Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia.
Guido Flamini Dipartimento di Farmacia, via Bonanno 6, 56126 Pisa, Italy ; Centro Interdipartimentale di Ricerca "Nutraceutica e Alimentazione per laSalute" Nutra food, University of Pisa, Italy.
Manel Ben M'hadheb Unité de Recherche “Génomique, Biotechnologie et Stratégies Antivirales”, Institut Supérieur de Biotechnologie, Université de Monastir, BP74, Avenue Tahar Hadded, 5000 Monastir, Tunisia.
safia El-BOK Laboratoire de Biodiversité, Biotechnologies & Changements Climatiques, Université Tunis El-Manar, Département de Biologie, Faculté des Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia. https://orcid.org/0000-0002-2987-9798
Lotfi Achour Laboratoire de Recherche “Bioressourses, Biologie Intégrative & Valorisation”, Institut Supérieur de Biotechnologie de Monastir, Tunisia.

DOI:

https://doi.org/10.56027/JOASD.072024

Keywords:

Opuntia microdasys Lehm., Opuntia macrorhiza Engelm., seeds, HS-SPME-GC/MS, polyphenols, Pharmacological activities

Abstract

The seeds of Opuntia sp. require additional investigations because they could contain several active molecules. Therefore, the present study analyzed the chemical composition and biological activities of Opuntia microdasys and that of Opuntia macrorhiza using in vitro and in vivo models.

The headspace of the powders of the seeds of Opuntia have been analyzed by gas chromatography–mass spectrometry (GC–MS). The analgesic activity was assessed using the acetic acid-induced abdominal constriction response on mouse. Chemical analyses showed that the powders of the seeds of O. microdasys and O. macrorhiza were rich in nonanal and camphor. Also, the O. macrorhiza seeds has a high antioxidant activity (EC₅₀ =0.12 mg/ml). The results showed that the ethanolic extracts, at a dose of 200 mg/kg of body weight, caused a significant (P< 0.05) inhibition of the abdominal constriction response (75.17% and 56.22% for O. microdasys and O. microdasys). The microdilution assay revealed that ethanolic extract of O. macrorhiza seeds is more active and could inhibit the growth of almost all the evaluated bacterial and fungal strains, with the minimal bactericidal concentration values (MIC) ranging from 0.039 to 2.50 mg/mL.

The overall results highlighted the potential of the tested Opuntia seeds that can be used as a source of bioactive compounds that confer various important biological activities to it, which could imply other pharmacological applications.

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