Genetic diversity and relationships among wild and cultivated Ficus carica L.: Usefulness of RGA markers

Sahar Haffar; Ghada Baraket; Gabriele Usai; Aymen Aounallah; Ahmed Ben Abdelkrim; Khaled Chatti; Amel Salhi Hannachi

doi:10.56027/JOASD.072023

Authors

Sahar Haffar
Ghada Baraket
Gabriele Usai
Aymen Aounallah
Ahmed Ben Abdelkrim
Khaled Chatti
Amel Salhi Hannachi

DOI:

https://doi.org/10.56027/JOASD.072023

Keywords:

Ficus carica L., RGA, Tunisia, structuring, genetic diversity

Abstract

Disease resistance and the maintenance of genetic diversity in wild and cultivated populations are very important challenges to implement breeding program and markers assisted selection of Ficus carica L. facing climate change and its consequences. Resistance gene analogs (RGA) markers were used for variety discrimination and assessment of genetic structure and diversity of wild and cultivated Ficus carica L. species in Tunisia. The RGA markers were efficient and reliable markers for discriminating wild and cultivated fig. The high level of polymorphism (95.65) detected suggests the effectiveness of RGAs for both genetic fingerprinting and relationships assessment in wild and cultivated fig. The detected markers may represent candidate genes for disease resistance and could be further used to facilitate the identification of candidate genes and accelerate the genetic improvement of disease resistance in breeding programs of Ficus carica species.

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