Molecular characterization of some Tunisian biferous fig accessions (Ficus carica L.) using RAPD and ISSR markers

Fateh Aljane; Mohamed Hichem Neily; Awatef Essid; Hajer Abou El Farah

doi:10.56027/JOASD082025

Authors

Fateh Aljane IRA Medenine
Mohamed Hichem Neily
Awatef Essid
Hajer Abou El Farah

DOI:

https://doi.org/10.56027/JOASD082025

Keywords:

Ficus carica, Biferous Fig accessions, Genetic diversity, RAPD, ISSR markers

Abstract

This work studied the evaluation of the genetic diversity of twelve biferous Tunisian fig accessions ('Bither Akhal,' 'Bither Ahmer1', 'Bither4', 'Bither Ahmer2', 'Bither2', 'Bither3', 'Bither1', 'Besbessi,' 'Wahchi,' 'Khenziri,' 'Garghi' and 'Bouholi') using molecular markers (RAPD and ISSR) and to determine the relatedness between accessions. Among the 31 DNA loci, 29 were polymorphic (93.61 %). With primers OPL11 and OPW04, DNA fragments produced a minimum of 8 and a maximum of 11 polymorphic bands. Primers had a collective resolving power value (Rp) of 13. Regarding ISSR markers, 47 DNA loci were identified, with 41 loci (87.24 %) being polymorphic. Polymorphic DNA fragment bands ranged from 8 (AM5) to 14 (AM6). Primers revealed 14.8 collective resolving power values (Rp). For RAPD and ISSR markers, the 'Bither3' and 'Bither4' accessions showed the highest genetic distances. UPGMA generated independent dendrograms based on RAPD and ISSR banding patterns. This study revealed significant genetic diversity among local fig accessions, indicating their potential as sources for breeding programs in fig selection.

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