Assessment of the physio-biochemical performance of Tunisian barley landraces under deficit saline-irrigation during grain filling stage

Mohamed  Bagues; Mohamed  Neji; Jaleh Ghashghaie; Tebra Triki; Ferdaous Guesmi; Nissaf  Karbout; Talel Bouhamda; Kamel Nagaz

doi:10.56027/JOASD.082022

Authors

Mohamed Bagues
Mohamed Neji
Jaleh Ghashghaie
Tebra Triki
Ferdaous Guesmi
Nissaf Karbout
Talel Bouhamda
Kamel Nagaz

DOI:

https://doi.org/10.56027/JOASD.082022

Keywords:

Barley, Δ13C, Phenolic Compounds, Gas Exchange, Photosynthetic pigments

Abstract

Salinity is one of the main and important abiotic stresses that adversely affects crop growth, development and production. In this study, two barley (Hordeum vulgare L.) landraces were subjected to three treatments of deficit saline-irrigation (12 dS/cm) (T0 = 100%ETc, T1 = 75%ETc, and T2 = 50%ETc) during grain filling stage. Carbon isotope discrimination (Δ¹³C) was associated with some physio-biochemical parameters to evaluate barley response to saline conditions. Results of this study showed that deficit saline-irrigation significantly (p < 0.05) decreases Δ¹³C in both barley landraces. Moreover, photosynthetic rate (A), transpiration (E), stomatal conductance (gs), and instantaneous water use efficiency (iWUE) were significantly affected by treatments. Relative water content (RWC), chlorophyll a, and chlorophyll (SPAD) value were significantly (p < 0.01 and p < 0.001) were affected by deficit saline-irrigation. In addition, phenolic compounds were affected by treatments and landraces (except syringic and p-coumaric acids), and their interactions (except syringic acid). Moreover, high correlations were noticed between Δ¹³C and physio-biochemical parameters. Results suggested that both barley landraces make a higher iWUE, and a weak variation in phenolic compounds. Moreover, Δ¹³C associated with physio-biochemical traits can also be good criteria for screening of salt-tolerance of barley during grain filling stage. Taken together, our study suggests that the response to deficit saline-irrigation in barley landraces involves an interplay between various physiological and biochemical mechanisms mainly related to Δ¹³C.

References

Anwar, S, Shafi., M, Bakht., J, Jan., MT, Hayat., Y. (2011). Response of barley genotypes to salinity stress as alleviated by seed priming. Pakistan Journal of Botany 43, 2687–2691.

Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiology 24, 1–15.

Ashrafi, E., Razmjoo, J., Zahedi, M., Pessarakli, M. (2014). Selecting alfalfa cultivars for salt tolerance based on some physiochemical traits. Agronomy Journal 106, 1758–1764.

Bagues, M., Hafsi, C., Yahia, Y., Souli, I., Boussora, F., Nagaz, K. (2019). Modulation of photosynthesis, phenolic contents, antioxidant activities, and grain yield of two barley accessions grown under deficit irrigation with saline water in the arid area of Tunisia. Polish Journal of Environmental Studies 28, 3071–3080.

Bagues, M., Zaghdoud, C., Hafsi, C., Boussora, F., Triki, T., Nagaz, K. (2020). Combined effect of deficit irrigation with saline water affects gas exchange, phytochemical profiles, antioxidant activities and grain yield of barley landraces “Ardhaoui” at heading stage. Plant Biosystems 1, 436–446.

Bharti, K., Pandey, N., Shankhdhar, D., Srivastava, P.C., Shankhdhar, S.C. (2014). Effect of different zinc levels on activity of superoxide dismutases & acid phosphatases and organic acid exudation on wheat genotypes. Physiology and Molecular Biology of Plants 20, 41–48.

Bonales-Alatorre, E., Shabala, S., Chen, Z.H., Pottosin, I. (2013). Reduced tonoplast fast activating and slow activating channel activity is essential for conferring salinity tolerance in a facultative halophyte, quinoa. Plant Physiology 162, 940–952.

Ceccarelli, S., Grando, S., Baum, M. (2007). Participatory plant breeding in water-limited environments. Experimental Agriculture 43, 411–435.

Chaves, M.M., Flexas, J., Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annal of Botany 103, 551–560.

Condon, A.G., Richards, R.A., Farquhar, G.D. (1993). Relationships between carbon isotope discrimination, water-use efficiency and transpiration efficiency for dryland wheat. Australian Journal of Agricultural Research 4, 1693–1711.

Dadkhah, A. (2013). Effect of salinity on carbon isotope discrimination of shoot and root of Four Sugar Beet (Beta vulgaris L.) Cultivars. Journal of Agricultural Science and Technology 15, 901–910

Eisa, S., Hussin, S., Geissler, N., Koyro, H. (2012). Effect of NaCl salinity on water relations, photosynthesis and chemical composition of Quinoa (Chenopodium quinoa Willd.) as a potential cash crop halophyte. Australian Journal of Crop Science 6, 357–368

El-Wahed, M.A., Sabagh, A.E., Saneoka, H., Abdelkhalek, A., Barutçular, C. (2015). Sprinkler irrigation uniformity and crop water productivity of barley in arid region. Emirates Journal of Food and Agriculture 27, 770–775.

Gao, Q., Sun, J., Tong, H., Wang, W., Zhang, Y., Zhang, G., Ma, D., Chen, W. (2018). Evaluation of rice drought stress response using carbon isotope discrimination. Plant Physiology and Biochemistry 132, 80–88.

Georgiev, V., Ananga, A., Tsolova, V. (2014). Recent advances and uses of grape flavonoids as nutraceuticals. Nutrients 6, 391–415.

Ghoulam, C., Fares, K. (2001). Effect of salinity on seed germination and early seedling growth of sugar beet (Beta vulgaris L.). Seed. Science and Technology 29, 357–364.

Gill, S.S., Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48, 909–930.

Goussi, R., Manaa, A., Derbali, W., Cantamessa, S., Abdelly, C., Barbato, R. (2018). Comparative analysis of salt stress, duration and intensity, on the chloroplast ultrastructure and photosynthetic apparatus in Thellungiella salsuginea. Journal of Photochemistry and Photobiology B 183, 275–287.

Hafsi, C., Falleh, H., Saada, M., Ksouri, R., Abdelly, C. (2017). Potassium deficiency alters growth, photosynthetic performance, secondary metabolites content, and related antioxidant capacity in Sulla carnosa grown under moderate salinity. Plant Physiology and Biochemistry 118, 609–617.

Hafsi, C., Falleh, H., Saada, M., Rabhi, M., Mkadmini, K., Ksouri, R., Abdelly, C., Smaoui, A. (2016). Effects of potassium supply on growth, gas exchange, phenolic composition, and related antioxidant properties in the forage legume Sulla carnosa. Flora 223, 38–45.

Hamid, M., Ashraf, M.Y., Khalil-ur-Rehman, Arashad, M. (2008). Influence of salicylic acid seed priming on growth and some biochemical attributes in wheat grown under saline conditions. Pakistan Journal of Botany 40, 361–367

Hosseinzadeh, S.R., Amiri, H., Ismaili, A. (2018). Evaluation of photosynthesis, physiological, and biochemical responses of chickpea (Cicer arietinum L. cv. Pirouz) under water deficit stress and use of vermicompost fertilizer. Journal of Integrative Agriculture 17, 2426–2437.

Jamalian, S., Gholami, M., Esna-Ashari, M. (2013). Abscisic acid-mediated leaf phenolic compounds, plant growth and yield is strawberry under different salt stress regimes. Theoretical and Experimental Plant Physiology 25, 291–299.

Jamil, A., Riaz, S., Ashra, f-M., Foolad, M.R. (2011). Gene expression profiling of plants under salt stress. Critical Reviews in Plant Sciences 30, 435–458.

Jiang, C., Zu, C., Lu, D., Zheng, Q., Shen, J., Wang, H., Li, D. (2017). Effect of exogenous selenium supply on photosynthesis, Na+ accumulation and antioxidative capacity of maize (Zea mays L.) under salinity stress. Scientific Reports 7, 42039.

Jiang, Q., Roche, D., Monaco, T.A., Durham, S. (2006). Gas exchange, chlorophyll fluorescence parameters and carbon isotope discrimination of 14 barley genetic lines in response to salinity. Field Crops Research 96, 269–278.

Karuppanapandian, T., Moon, J.C., Kim, C., Manoharan, K., Kim, W. (2011). Reactive oxygen species in plants: their generation, signal transduction, and scavenging mechanisms. Australian Journal of Crop Science 5, 709–725

Keller, K.M., Lienert, S., Bozbiyik, A., Stocker, T.F., Churakova (Sidorova), V.O., Frank, D.C., Klesse, S., Koven, C.D., Leuenberger, M., Riley, W.J., Saurer, M., Siegwolf, R., Weigt, R.B., Joos, F. (2017). 20th century changes in carbon isotopes and water-use efficiency: tree-ring-based evaluation of the CLM4.5 and LPX-Bern models. Biogeosciences 14, 2641–2673.

Khazaei, H., Mohammady, S.D., Zaharieva, M., Monneveux, P. (2008). Carbon isotope discrimination and water use efficiency in Iranian diploid, tetraploid and hexaploid wheat grown under well-watered conditions. Genetic Resources and Crop Evolution 56, 105–114.

Koyro, H.W. (2006). Effect of salinity on growth, photosynthesis, water relations and solute composition of the potential cash crop halophyte Plantago coronopus (L.). Environmental and Experimental Botany 56, 136–146.

Manaa, A., Goussi, R., Derbali, W., Cantamessa, S., Abdelly, C., Barbato, R. (2019). Salinity tolerance of quinoa (Chenopodium quinoa Willd.) as assessed by chloroplast ultrastructure and photosynthetic performance. Environmental and Experimental Botany 162, 103–114.

Mansour, E., Moustafa, E.S.A., Desoky, E-S.M., Ali, M.M.A., Yasin, M.A.T., Attia, A., Alsuhaibani, A., Tahir, M.U., El-Hendawy, S. (2020). Multidimensional evaluation for detecting salt tolerance of bread wheat genotypes under actual saline field growing conditions. Plants 9, 1324.

Mbarki, S., Sytar, O., Cerda, A., Zivcak, M., Rastogi, A., He, X., Zoghlami, A., Abdelly, C., Brestic, M. (2018). Strategies to Mitigate the Salt Stress Effects on Photosynthetic Apparatus and Productivity of Crop Plants. In: Kumar V, Wani S, Suprasanna P, Tran LS. (eds) Salinity Responses and Tolerance in Plants, Volume 1. Springer, Cham https://doi.org/10.1007/978-3-319-75671-4_4

Monneveux, P., Rekika, D., Acevedo, E., Merah, O. (2006). Effect of drought on leaf gas exchange, carbon isotope discrimination, transpiration efficiency and productivity in field grown durum wheat genotypes. Plant Science 170, 867–872.

Moussa, Z., Judeh, Z.M.A., Ahmed, S.A. (2019). Nonenzymatic Exogenous and Endogenous Antioxidants. Free Radical Medicine and Biology [Working Title]. https://doi.org/10.5772/intechopen.87778

Munns, R., Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology 59, 651–681.

Mustafa, A., Mahmood, K., Ishaque, W. (2019). Evaluation of salt tolerance and its relationship with carbon isotope discrimination and physiological parameters of barley genotypes. Communications in Soil Science and Plant Analysis 50, 594–610.

Negrão, S., Schmöckel, S.M., Tester, M. (2017). Evaluating physiological responses of plants to salinity stress. Annals of Botany 119, 1–11.

Netondo, G.W., Onyango, J.C., Beck, E. (2004). Sorghum and salinity: II. Gas exchange and chlorophyll fluorescence of sorghum under salt stress. Crop Science 44, 806–811.

Pirasteh-Anosheh, H., Ranjbar, G., Pakniyat, H. (2016). Chapter 8: Physiological mechanisms of salt stress tolerance in plants: an overview. In: Mahgoub Azooz M, Ahmad P, editors. Plant–environment interaction: responses and approaches to mitigate stress. Jammu and Kashmir: John Wiley & Sons, Ltd p:141–160. https://doi.org/10.1002/9781119081005.ch8

Rasool, A., Shah, W.H., Tahir, I., Alharby, H.F., Hakeem, K.R., Rehman, R. (2020). Exogenous application of selenium (Se) mitigates NaCl stress in proso and foxtail millets by improving their growth, physiology and biochemical parameters. Acta Physiologiae Plantarum 42, 116.

Rouhi, V., Samson, R., Lemeur, R., Van Damme, P. (2007). Photosynthetic gas exchange characteristics in three different almond species during drought stress and subsequent recovery. Environmental and Experimental Botany 59, 117–129.

Sarabi, B., Bolandnazar, S., Ghaderi, N., Ghashghaie, J. (2017). Genotypic differences in physiological and biochemical responses to salinity stress in melon (Cucumis melo L.) plants: prospects for selection of salt tolerant landraces. Plant Physiology and Biochemistry 119, 294–311.

Sarabi, B., Fresneau, C., Ghaderi, N., Bolandnazar, S., Streb, P., Badeck, F-W., Citerne, S., Tangama, M., David, A., Ghashghaie, J. (2019). Stomatal and non-stomatal limitations are responsible in down-regulation of photosynthesis in melon plants grown under the saline condition: Application of carbon isotope discrimination as a reliable proxy. Plant Physiology and Biochemistry 141, 1–19.

Sayyad-Amin, P., Borzouei, A., Jahansooz, M.R., Ajili, F. (2016). Changes in photosynthetic pigments and chlorophyll-a fluorescence attributes of sweet-forage and grain sorghum cultivars under salt stress. Journal of Biological Physics 42, 601–620.

Shahbaz, M., Ashraf, M., Al-Qurainy, F., Harris, P.J.C. (2012). Salt tolerance in selected vegetable crops. Critical Reviews in Plant Sciences 31, 303–320.

Shaheen, R., Hood-Nowotny, R.C. (2005). Effect of drought and salinity on carbon isotope discrimination in wheat cultivars. Plant Science 168, 901–909.

Sharp, R.E., Hsiao, T.C., Silk, W.K. (1990). Growth of the maize primary root at low water potentials: II. role of growth and deposition of hexose and potassium in osmotic adjustment. Plant Physiology 93, 1337–1346.

Shirazi, M.U., Khan, M.A., Mujtaba, S.M., Shereen, A., Hood, R.C., Mayr, L., Khan, M.A., Mahboob, W. (2015). Evaluation of salt tolerance in wheat genotypes on growth and carbon isotopes discrimination technique. Pakistan Journal of Botany 47, 829–33.

Skrovankova, S., Sumczynski, D., Mlcek, J., Jurikova, T., Sochor, J. (2015). Bioactive compounds and antioxidant activity in different types of berries. International Journal of Molecular Sciences 16, 24673–24706

Stagnari, F., Galieni, A., D’Egidio, S., Falcinelli, B., Pagnani, G., Pace, R., Pisante, M., Benincasa, P. (2017). Effects of sprouting and salt stress on polyphenol composition and antiradical activity of einkorn, emmer and durum wheat. Italian Journal of Agronomy 12, 848.

Subramanyam, K., Du Laing, G., Van Damme, E.J. (2019). Sodium selenite treatment using a combination of seed priming and foliar spray alleviates salinity stress in rice. Frontiers in Plant Science 10. https://doi.org/10.3389/fpls.2019.00116

Sun, Q., Yamada, T., Takano, T. (2014). Salinity effect on germination, growth, and photosynthesis, and ion accumulation in wild Miscanthus sinensis Anderss. Populations. Crop Science 54, 2760–2771.

Tsialtas, J.T., Maslaris, N. (2006). Leaf carbon isotope discrimination relationships to element content in soil, roots and leaves of sugar beets grown under Mediterranean conditions. Field Crops Research 99, 125–135.

Wang, Y., Sun, Y., Niu, G., Deng, C., Wang, Y., Gardea-Torresdey, J. (2019). Growth, gas exchange, and mineral nutrients of ornamental grasses irrigated with saline water. HORTSCIENCE 54, 1840–1846.

Zhao, G.Q., Ma, B.L., Ren, C.Z. (2007). Growth, gas exchange, chlorophyll fluorescence, and ion content of naked oat in response to salinity. Crop Science 47, 123–131.

Zheng, S., Cui, N., Gong, D., Wang, Y., Hu, X., Feng, Y., Zhang, Y. (2020). Relationship between stable carbon isotope discrimination and water use efficiency under deficit drip irrigation of kiwifruit in the humid areas of South China. Agricultural Water Management 240, 106300.