Polysaccharides from Tossa jute (Corchorus olitorius L.) leaves: Extraction, antioxidant and antibacterial activities


  • Amira Racha Ben Yakoub
  • Mourad Jridi
  • Walid Elfalleh
  • Ali Ferchichi




Tossa jute, Corchorus olitorius L., polysaccharides, antioxidant activities


The biological proprieties of mucilaginous polysaccharides (PSc) extracted from Tossa jute leaves (Corchorus olitorius  L.) using an ethanol precipitation method were studied. The results showed that PSc had higher total polyphenols and flavonoids and greater antioxidant activities. At 1.5 mg/ml PSc, the antioxidant activities were about 90% against 1,1-diphenyl-2-picryl hydrazyl radical (DPPH•) and 78% against lipid peroxidation. The PSc with a FRAP assay at the same concentration showed an effective protection against hydroxyl radicals and DNA breakage. Furthermore, the extracted PSc had a wide spectrum of antibacterial activities against all bacteria tested (Gram+ and Gram-). The overall data suggested that this natural PSc may be used as a competitive antioxidant and antimicrobial additive in food and in medicinal preparations.


Abdelhedi, O., Nasri, R., Souissi, N., Nasri, M., & Jridi, M. (2016). Sulfated polysaccharides from common smooth hound: Extraction and assessment of anti-ACE, antioxidant and antibacterial activities. Carbohydrate Polymers, 152, 605–614.

Andrade, D., Gil, C., Breitenfeld, L., Domingues, F., & Duarte, A. (2009). Bioactive extracts from Cistus ladanifer and Arbutus unedo L. Industrial Crops and Products, 30, 165–167.

AOAC. (2000). Official Methods of Analysis (17th ed.). Washington, DC, USA: Association of Official Analytical Chemists.

Azuma, K., Nakayama, M., Koshioka, M., Ippoushi, K., Yamaguchi, Y., Kohata, K., Yamauchi, Y., Ito, H., & Higashio, H. (1999). Phenolic antioxidants from the leaves of Corchorus olitorius L. Journal of Agricultural and Food Chemistry, 47, 3963–3966.

Barku, V.Y. A., Boahen, Y. O., Ansah, E. O., Dayie, N. T. K. D., & Mensah, F. E. (2013). In-vitro assessment of antioxidant and antimicrobial activities of methanol extracts of six wound healing medicinal plants. Journal of Natural Sciences Research, 3, 74–80.

Berghe, V. A., & Vlieinck, A. J. (1991). Screening methods for antibacterial and antiviral agents from higher plants Methods. Plant Biochemistry, 6(3), 47–68.

Bertani, G. (1951). Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. Journal of Bacteriology, 62, 293–300.

Ben Jeddou, K., Chaari, F., Maktouf, S., Nouri-Ellouz, O., BoissetHelbert, C., & Ellouz G. R. (2016). Structural, functional, and antioxidant properties of water-soluble polysaccharides from potatoes peels. Food Chemistry, 205, 97–105.

Ben Romdhane, M., Haddar, A., Ghazala, I., Ben Jeddou, K., Helbert, C. B., & Ellouz-Chaabouni S. (2017). Optimization of polysaccharides extraction from watermelon rinds: Structure, functional and biological activities. Food Chemistry, 216, 355–364.

Ben Yakoub, A. R., Abdehedi, O., Jridi, M., Elfalleh, W., Nasri, M., & Ferchichi, A. (2018). Flavonoids, phenols, antioxidant and antimicrobial activities in various extracts from Tossa jute leave (Corchorus olitorius L.). Industrial Crops & Products, 118, 206–213

Bersuder, P., Hole, M., & Smith, G. (1998). Antioxidants from a heated histidine–glucose model system. Investigation of the antioxidant role of histidine and isolation of antioxidants by high performance liquid chromatography. Journal of the American Oil Chemists Society, 75(2), 181–187.

Bitter, T., & Muir, H. M. (1962). A modified uronic acid carbazole rection. Analytical Biochemistry, 4(4), 330–334.

Challouf, R., Trabelsi, L., Ben Dhieb, R., El Abed, O., Yahia, A., Ghozzi K., Ben Ammar, J., Omran, H., & Ben Ouada, H. (2011). Evaluation of cytotoxicity and biological activities in extracellular polysaccharides released by Cyanobacterium Arthrospira platensis. Brazilian Archive of Biology and Technology, 54(3), 831–838.

Dong, H., Zhang, Q., Li, Y., Li, L., Lan, W., He, J., Li, H., Xiong, Y., & Qin, W. (2016). Extraction, characterization and antioxidant activities of polysaccharides of Chuanminshen violaceum. International Journal of Biological Macromolecules, 86, 224–232.

Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350–356.

Furumoto T., Wang R., Okazaki K., & Fukui, H. (2002). Antitumor promoters in leaves of jute (Corchorus capsularis and Corchorus olitorius ). Food Science and Technology Research, 8(3), 239–243.

Ghazala, I., Sila, A., Frikha, F., Driss, D., Ellouz-Chaabouni, S., & Haddar, A. (2015). Antioxidant and antimicrobial properties of water soluble polysaccharide extracted from carrot peels by-products. Journal of Food Science and Technology, 52(11), 6953–6965.

He, F., Yang, Y., Yang, G., & Yu, L. (2010). Studies on antibacterial activity and antibacterial mechanism of a novel polysaccharide from Streptomyces virginia H03. Food Control, 21(9), 1257–1262.

Hseu, Y. C., Chang, W. H ., Chen, C. S., Liao, D. J. W, Huang, C. J., Lu, F. J., Chia, Y. C., Hsu, H. K., Wu, J. J., & Yang, H. L. (2008). Antioxidant activities of Toona sinensis leaves extracts using different antioxidant models. Food and Chemical Toxicology, 46(1), 105–114.

Hussein, M. M., Hassan, F. A. M., Abdel Daym, H. H., Salama, A., Enab, A. K., & Abd El-Galil, A. A. (2011). Utilization of some plant polysaccharides for improving yoghurt consistency. Annals of Agricultural Sciences, 56(2), 97–103.

Ignat, C. M., & Desbrieres, J. (2012). Compatibilité et co-structuration dans des systèmes contenant des scléroprotéines et des polysaccharides. Thesis in Polymer Chemistry (Pau, Pyrenees Atlantiques). Université de Pau et des Pays de l'Adour, France https://scanr.enseignementsuprecherche.gouv.fr/publication/these2012PAUU3017 .

Javanmardi, J., Stushnoff, C., Locke, E., & Vivanco J. M. (2003). Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry. 83(4), 547–550.

Kil, H. Y., Seong, E. S., Ghimire, B. K., Chung, I. M., Kwon, S. S., Goh, E. J., & Yu, C. Y. (2009). Antioxidant and antimicrobial activities of crude sorghum extract. Food Chemistry, 115(4), 1234–1239.

Koleva, I. I., Van Beek, T. A., Linssen, J. P. H., Groot, A., & Evstatieva, L. N. (2002). Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochemical Analysis, 13(1), 8–17.

Lee, J., Kim, H., Kim, J., & Jang, Y. (2002). Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. Saboten. Journal of Agricultural and Food Chemistry, 50(22), 6490–6496.

Li, J. E., Wanga, W. J., Zhenga, G. D., & Lib, L.Y. (2017). Physicochemical properties and antioxidant activities of polysaccharides from Gynura procumbens leaves by fractional precipitation. International Journal of Biological Macromolecules, 95, 719–724.

Lin, J. Y., & Tang C. Y. (2007). Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chemistry, 101(1), 140–147.

Liu, Q., Tian, G., Yan, H., Geng, X., Cao, Q., Wang, H., & Nag, T. B. (2014). Characterization of polysaccharides with antioxidant and hepatoprotective activities from the wild edible mushroom Russula vinosa Lindblad. Journal of Agricultural and Food Chemistry, 62(35), 8858–8866.

Liu, X., Sun, Z., Zhang, M., Meng, X., Xia, X., Yuan, W., Xue, F., & Liu, C. (2012). Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber. Apostichopus japonicus. Carbohydrate Polymers, 90(4), 1664–1670.

Lovegrovea, C. H., Edwardsb, I., De NonicPatelb, H., Eld, S.N., Grassbyb, T., Zielkee, C., Ulmiusf, M., Nilssone, L., Butterworthb, P. J., Ellisb, P. R ., & Shewry, P. R. (2017). Role of polysaccharides in food, digestion and health. Critical Reviews in Food Science and Nutrition, 57(2), 237–253.

Ma, L., Chen, H., Zhang, Y., Zhang, N., & Fu, L. (2012). Chemical modification and antioxidant activities of polysaccharide from mushroom Inonotus obliquus. Carbohydrate Polymers, 89(2), 371–378.

Majdoub, H., Mansour, M. B., Chaubet, F., Roudesli, M. S., & Maaroufi, R. M. (2009). Anticoagulant activity of a sulfated polysaccharide from the green alga Arthrospira platensis. Biochimica et Biophysica Acta, 1790(10), 1377–1381.

Namki, M. (1990). Antioxidants/antimutagens in food. Critical Reviews in Food Science and Nutrition. 29(4), 273-300.

Oboh, G., Raddatz, H., & Henle, T. (2009). Characterization of the antioxidant properties of hydrophilic and lipophilic extracts of jute (Corchorus olitorius ) leaf. International Journal of Food Science and Nutrition, 60(2), 124–134

Ohtani, K., Okai, K., Yamashita, U., Yuasa, I., & Misaki, A. (1995). Characterization of an acidic polysaccharide isolated from the leaves of Corchorus olitorius (Moroheiya). Bioscience, Biotechnology and Biochemistry, 59(3), 378–381.

Osawa, T., & Namiki, M. (1985). Natural antioxidants isolated from eucalyptus leaf waxes. Journal of Agricultural and Food Chemistry, 33(5), 777–780.

Quang, V. N., & Jong-Bang, E. (2011). Antioxidant activity of solvent extracts from Vietnamese medicinal plants. Journal of Medicinal Plants Research, 5(13), 2798–2811.

Ramadevi, D., & Ganapaty, S. (2011). Antimicrobial activity of Corchorus olitorius L. Pharmacologyonline, 2, 1303-1308.

Raposo, M. P. F. J., Morais, R. M. S. C., & Morais, A. M. M .B. (2013). Bioactivity and applications of sulphated polysaccharides from marine microalgae. Marine Drugs, 11(12), 233–252.

Sila, A., Bayar, N., Ghazala, I., Bougatef, A., Ellouz-Ghorbel, R., & Ellouz-Chaabouni, S. (2014). Water-soluble polysaccharides from agro-industrial by-product: Functional and biological properties. International Journal of Biological Macromolecules, 69, 236–243.

Xie, J. H., Wang, Z. J., Shen, M. Y., Nie, S. P., Gong, B., Li, H. S., Zhao, Q., Li, W. J., & Xie, M.Y. (2015). Sulfated modification, characterization and antioxidant activities of polysaccharide from Cyclocarya paliurus. Food Hydrocolloids, 53, 7–15

Xua, Z., Wanga, H., Wanga, B., Fub, L., Yuana, M., Liua, J., Zhoua, L., & Chunbang, D. (2016). Characterization and antioxidant activities of polysaccharides from the leaves of Lilium lancifolium Thunb. International Journal of Biological Macromolecules, 92, 148–155

Yildirim, A., Mavi, A., & Kara, A. A. (2001). Determination of antioxidant and antimicrobial activities of Rumexcrispus L. extracts. Journal of Agricultural and Food Chemistry, 49(8), 4083–4089.

Zhu, B. W., Wang, L. S., Zhou, D.Y., Li, D. M., Sun, L. M, Yang, J. F., Wu, H. T., Zhou, X. Q., & Tada, M. (2008). Antioxidant activity of sulphated polysaccharide conjugates from abalone (Haliotis discus hannai Ino). European Food Research and Technology, 227(6), 1663–1668.

Zeghichi, S., Kallithraka, S., & Simopoulos, A. P. (2003).Nutritional composition of molokhia (Corchorus olitorius ) and stamnagathi (Cichorium spinosum).World Review of Nutrition and Dietetics, 91, 1–21.




How to Cite

Ben Yakoub, A. R., Jridi, M., Elfalleh, W., & Ferchichi, A. (2020). Polysaccharides from Tossa jute (Corchorus olitorius L.) leaves: Extraction, antioxidant and antibacterial activities. JOURNAL OF OASIS AGRICULTURE AND SUSTAINABLE DEVELOPMENT, 4(2), 231–238. https://doi.org/10.56027/JOASD.spiss312022

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