Morphological and physiological behaviour of sea barley (Hordeum marinum ssp marinum)  genotypes originating from Soliman Sebkha under increasing salinity

Warda Saoudi; Wael Taamalli; Chedly Abdelly; Mounawer Badri

doi:10.56027/JOASD.102023

Authors

Warda Saoudi
Wael Taamalli
Chedly Abdelly
Mounawer Badri

DOI:

https://doi.org/10.56027/JOASD.102023

Keywords:

Hordeum marinum, photosynthesis activity, salt tolerance indices, salinity stress

Abstract

Evaluation of germplasm in salt stress environment may help identifying salt-tolerant genotypes. In this study, we analyzed the genetic diversity of Hordeum marinum, genotypes originated from Soliman Sebkha in response to salinity (0, 250 and 400 mM) by measuring a set of parameters related to growth, water statue and photosynthetic activity. Results of ANOVA showed that variability of measured parameters was explained by the effects genotypes, treatment and their interaction, with treatment factor recorded the highest values. Salinity significantly reduced the photosynthetic rate and related parameters, including stomatal conductance (gs), intercellular CO2 concentration (Ci), and transpiration (E). Several indices for salt tolerance indices comprising tolerance index (TOL), stress tolerance index (STI) and Salt Tolerance (ST) were calculated based on drought biomass (ADW) under non-stressed and under stress conditions. Results showed that lower values of TOL and higher values of ST and STI indicate the superiority of the genotypes having enough plasticity to respond to extreme conditions. Principal Component Analysis clustered genotypes into sensitive (21.43%), moderately tolerant (50%), and highly tolerant (28.57%) groups, depending on their relative potential to sustain good growth under high salinity. Results indicating sufficient genetic variability for salinity tolerance among the studied genotypes, hence tolerant genotypes can be good candidates in future breeding programs.

References

Ananda, G.K.S., Myrans, H., Norton, S.L., Gleadow, R., Furtado, A., Henry, R.J.(2020).Wild Sorghum as a Promising Resource for Crop Improvement. Frontiers in Plant Science 11, 1108.

Ashraf, M., Harris, P.J.C. (2013). Photosynthesis under stressful environments: An overview. Photosynthetica, 51, 163–190.

Coyne, C.J., Kumar, S., Wettberg, E.J.B., Marques, E., Berger, J.D., Redden, R.J., Ellis, T.H.N. Brus, J., Zablatzká, L., Smýkal, P. (2020) Potential and limits of exploitation of crop wild relatives for pea, lentil, and chickpea improvement. Legume Science 2, e36.

Debez, A., Koyro, H.W., Grignon, C., Abdelly, C., Huchzermeyer, B. (2008). Relationship between the photosynthetic activity and the performance of Cakile maritima after long-term salt treatment. Physiologia Plantarum 133, 373-385.

Fernandez, G.C.J. (1992) Effective selection criteria for assessing stress tolerance. In Kuo CG (ed) Proceedings of the international symposium on adaptation of vegetables and other food crops in temperature and water stress, Taiwan, pp. 257–270.

Flowers, T.J., Flowers, S.A. (2005). Why does salinity pose such a difficult problem for plant breeders? Agricultural Water Management 78, 15–24.

Frazão, J.J., Prado, R.M., Júnior, J.P.S., Rossatto, D.R. (2020). Silicon changes C:N:P stoichiometry of sugarcane and its consequences for photosynthesis, biomass partitioning and plant growth. Scientific Reports 10:12492. https:// doi. org/ 10. 1038/ s41598- 020- 69310-6

Genc, Y., McDonald, G.K., Tester, M. (2007). Reassessment of tissue Na concentration as a criterion for salinity tolerance in bread wheat. Plant, Cell & Environment 30,1486–1498

Hafsi, C., Lakhdar, A., Rabhi, M., Debez, A., Abdelly, C., Ouerghi, Z. (2007). Interactive effects of salinity and potassium availability on growth, water status, and ionic composition of Hordeum maritimum. Journal of Plant Nutrition and Soil Science. 170, 469-473.

Hewitt, E.J. (1966). Sand and water culture methods used in the study of plant nutrition. Commonwealth Bureau of Horticulture, Technical Communication, 22, 431–446.

Isayenkov, S., Hilo, A., Rizzo, P., Moya, T .A.Y., Rolletschek, H., Borisjuk, L., Radchuk, V. (2020). Adaptation Strategies of Halophytic Barley Hordeum marinum ssp. marinum to High Salinity and Osmotic Stress. International Journal of Molecular Sciences, 21(23), 9019.

Jha, S., Singh, J., Chouhan, C., Singh, O., Srivastava, R. K. (2022). Evaluation of Multiple Salinity Tolerance Indices for Screening and Comparative Biochemical and Molecular Analysis of Pearl Millet [Pennisetum glaucum (L.) R. Br.] Genotypes. Journal of Plant Growth Regulation 41, 1820–1834.

Kwon, O.K., Mekapogu, M., Kim, K.S. (2019). Efect of salinity stress on photosynthesis and related physiological responses in carnation (Dianthus caryophyllus).Horticulture, Environment, and Biotechnology) 60, 831–839.

Maxted, N., Avagyan, A., Frese, L., Iriondo, J., Kell, S., Brehm, J.M., Singer, A., Dulloo, M.E.(2015). Conservation Planning for Crop Wild Relative Diversity. In Crop Wild Relatives and Climate Change; Wiley-Blackwell: Hoboken, NJ, USA, pp. 88–107.

Morton, M.J.L., Awlia, M., Al-Tamimi, N., Saade,S., Pailles, Y., Negrão, S., Tester, M. (2019). Salt stress under the scalpel – dissecting the genetics of salt Tolerance. Plant Journal 97,148–163.

Munns, R., James, R.A. (2003). Screening methods for salinity tolerance: case study with tetraploid wheat. Plant and Soil 253, 201-218.

Negrão, S., Schmӧckel, S. M., & Tester, M. (2017). Evaluating physiological responses of plants to salinity stress. Annals of Botany, 119, 1-11.

Nevo, E, Chen, G. (2010). Drought and salt tolerances in wild relatives for wheat and barley improvement. Plant, Cell & Environment 33, 670–685.

R, Core Team. (2020). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/.

Rosielle, A.A., Hamblin, J. (1981). Theoretical aspects of selection for yield in stress and non-stress environments. Crop Science 21, 943–946.

Saoudi, W., Badri, M., Gandour, M., Smaoui, A., Abdelly, C., Taamalli, W. (2019b). Analysis of genetic diversity and spatial structure in Tunisian populations of Hordeum marinum ssp. marinum based on molecular markers. The Journal of Agricultural Science 157 (5), 399–412.

Saoudi, W., Badri, M., Gandour, M., Smaoui, A., Abdelly, C., Taamalli, W. (2017). Assessment of genetic variability among Tunisian populations of Hordeum marinum using morpho-agronomic traits. Crop Science 57, 302–309.

Saoudi, W., Badri, M., Taamalli, W., Zribi, T.O., Gandour, M., Abdelly, C. (2019a). Variability in response to salinity stresss in natural Tunisian populations of Hordeum marinum subsp. marinum. Plant Biology 21(1), 89–100.

Siddiqui, M.H., Mohammad, F., Khan, M.N. (2009). Morphological and physio-biochemical characterization of Brassica juncea L.Czern. & Coss.genotypes under salt stress. Journal of Plant Interactions 4, 67-80.

Singh, S., Sengar, R.S., Kulshreshtha, N., Datta, D., Tomar, R.S., Rao, V.P., Garg, D., Ojha, A. (2015). Assessment of multiple tolerance indices for salinity stress in bread wheat (Triticum aestivum L). The Journal of Agricultural Science 7(3), 49–57.

Steduto, P., Albrizio, R., Giorio, P., Sorrentino, G. (2000). Gas exchange response and stomatal and non-stomatal limitations to carbon assimilation of sunfower under salinity. Environmental and Experimental Botany 44, 243–255.

Talbi, O.Z., Labidi, N., Slama, I., Debez, A., Ksouri, R., Rabhi, M., Smaoui, A., Abdelly, C. (2012). Alleviation of phosphorus deficiency stress by moderate salinity in the halophyte Hordeum maritimum L. Plant Growth Regulation 66, 75–85.

Wu, H, M., Zhu, L., Shabala, Zhou, M., Shabala, S. (2015). K+ retention in leaf mesophyll, an overlooked component of salinity tolerance mechanism: A case study for barley. Journal of integrative plant biology, 57(2), 171-185.

Yousfi, S., Rabhi, M., Hessini, K., Abdelly, C., Gharsalli, M. (2010). Differences in efficient metabolite management and nutrient metabolic regulation between wild and cultivated barley grown at high salinity. Plant Biology, 12, 650–658.

Zahraa,N., Al Hinaib, S.M., Hafeez, M.B., Rehman, A., Wahid, A., Siddique K.H.M., Farooq, M. (2022). Regulation of photosynthesis under salt stress and associated tolerance mechanisms. Plant Physiology and Biochemistry 178, 55-69.